Studies on Succinate Dehydrogenase. 8alpha-Histidyl-FAD as the Active Center of Succinate Dehydrogenase
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چکیده
منابع مشابه
Studies on Succinate Dehydrogenase
1. Succinate dehydrogenase flavocoenzyme (“SD-flavin”), previously shown to be an SOLsubstituted riboflavin derivative containing a tertiary nitrogen homoconjugated to the flavin nucleus, was subjected to further hydrolysis and to reduction under acid conditions. Both conditions resulted in the liberation of 1 mole of histidine per mole of flavin. This proves histidine to be the covalent link b...
متن کاملStudies on the binding of carboxin analogs to succinate dehydrogenase.
Carboxin (5,6-dihydro-2-methyl-l,4-oxathiin-3-carboxanilide) and a number of other carboxanilides are known to be potent inhibitors of succinate oxidation in membrane preparations from animal tissues, fungi, and bacteria, acting at the succinate dehydrogenase-coenzyme Q junction. The kinetics and the maximal extent of inhibition of succinate oxidation by carboxanilides and by thenoyltrifluoroac...
متن کاملSingle motoneuron succinate dehydrogenase activity.
We have developed a quantitative histochemical assay for measurement of succinate dehydrogenase (SDH) activity in single motoneurons. A computer image processing system was used to quantify the histochemical enzyme reaction product and to follow the time course of the reaction. The optimal concentration for each of the ingredients of the incubation medium for the SDH reaction was determined and...
متن کاملThe synthesis of succinate-dehydrogenase riboflavin.
Singer and Kearney"], in their early studies, recognized that the flavocoenzyme of mitochondria1 succinate dehydrogenase ("SD-flavin") [**I was a derivative of flavin-adenine-dinucleotide which was covalently bound to the protein. In cooperation with Dr. Singer's team [***I, we have attempted to elucidate the structure of this coenzyme. In the first preliminary communication I21, we concluded f...
متن کاملThe Succinate Dehydrogenase of Escherichia coli
Using EPR spectroscopy to monitor the integrity of the enzyme, conditions have been established which allow specific immunoprecipitation of the succinate dehydrogenase complex of Escherichia coli. The enzyme complex precipitated from Lubrol PX-solubilized membranes by monospecific antiserum in the presence of a cocktail of protease inhibitors contains four polypeptides of apparent Mrs 71,000,26...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1972
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1972.tb01766.x